Positioning of subdomain IIId and apical loop of domain II of the hepatitis C IRES on the human 40S ribosome
Identifieur interne : 002831 ( Main/Exploration ); précédent : 002830; suivant : 002832Positioning of subdomain IIId and apical loop of domain II of the hepatitis C IRES on the human 40S ribosome
Auteurs : Elena Babaylova [Russie] ; Dmitri Graifer [Russie] ; Alexey Malygin [Russie] ; Joachim Stahl [Russie] ; Ivan Shatsky [Russie] ; Galina Karpova [Russie]Source :
- Nucleic Acids Research [ 0305-1048 ] ; 2009.
Abstract
The 5′-untranslated region of the hepatitis C virus (HCV) RNA contains a highly structured motif called IRES (Internal Ribosome Entry Site) responsible for the cap-independent initiation of the viral RNA translation. At first, the IRES binds to the 40S subunit without any initiation factors so that the initiation AUG codon falls into the P site. Here using an original site-directed cross-linking strategy, we identified 40S subunit components neighboring subdomain IIId, which is critical for HCV IRES binding to the subunit, and apical loop of domain II, which was suggested to contact the 40S subunit from data on cryo-electron microscopy of ribosomal complexes containing the HCV IRES. HCV IRES derivatives that bear a photoactivatable group at nucleotide A275 or at G263 in subdomain IIId cross-link to ribosomal proteins S3a, S14 and S16, and HCV IRES derivatized at the C83 in the apex of domain II cross-link to proteins S14 and S16.
Url:
DOI: 10.1093/nar/gkn1026
Affiliations:
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wicri:rule="url">Russie</country></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Nucleic Acids Research</title><idno type="ISSN">0305-1048</idno><idno type="eISSN">1362-4962</idno><imprint><publisher>Oxford University Press</publisher><date type="published">2009</date><date type="e-published">2009</date><biblScope unit="vol">37</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="1141">1141</biblScope><biblScope unit="page" to="1151">1151</biblScope></imprint><idno type="ISSN">0305-1048</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0305-1048</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract">The 5′-untranslated region of the hepatitis C virus (HCV) RNA contains a highly structured motif called IRES (Internal Ribosome Entry Site) responsible for the cap-independent initiation of the viral RNA translation. At first, the IRES binds to the 40S subunit without any initiation factors so that the initiation AUG codon falls into the P site. Here using an original site-directed cross-linking strategy, we identified 40S subunit components neighboring subdomain IIId, which is critical for HCV IRES binding to the subunit, and apical loop of domain II, which was suggested to contact the 40S subunit from data on cryo-electron microscopy of ribosomal complexes containing the HCV IRES. HCV IRES derivatives that bear a photoactivatable group at nucleotide A275 or at G263 in subdomain IIId cross-link to ribosomal proteins S3a, S14 and S16, and HCV IRES derivatized at the C83 in the apex of domain II cross-link to proteins S14 and S16.</div></front></TEI><affiliations><list><country><li>Russie</li></country><region><li>District fédéral central</li></region><settlement><li>Moscou</li></settlement><orgName><li>Université d'État de Moscou</li></orgName></list><tree><country name="Russie"><region name="District fédéral central"><name sortKey="Babaylova, Elena" sort="Babaylova, Elena" uniqKey="Babaylova E" first="Elena" last="Babaylova">Elena Babaylova</name></region><name sortKey="Graifer, Dmitri" sort="Graifer, Dmitri" uniqKey="Graifer D" first="Dmitri" last="Graifer">Dmitri Graifer</name><name sortKey="Karpova, Galina" sort="Karpova, Galina" uniqKey="Karpova G" first="Galina" last="Karpova">Galina Karpova</name><name sortKey="Karpova, Galina" sort="Karpova, Galina" uniqKey="Karpova G" first="Galina" last="Karpova">Galina Karpova</name><name sortKey="Karpova, Galina" sort="Karpova, Galina" uniqKey="Karpova G" first="Galina" last="Karpova">Galina Karpova</name><name sortKey="Malygin, Alexey" sort="Malygin, Alexey" uniqKey="Malygin A" first="Alexey" last="Malygin">Alexey Malygin</name><name sortKey="Shatsky, Ivan" sort="Shatsky, Ivan" uniqKey="Shatsky I" first="Ivan" last="Shatsky">Ivan Shatsky</name><name sortKey="Stahl, Joachim" sort="Stahl, Joachim" uniqKey="Stahl J" first="Joachim" last="Stahl">Joachim Stahl</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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